Microdosing Psilocybe Cubensis: A Scientific Review in the Modern Psychedelic Renaissance

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The resurgence of the modern psychedelic renaissance has revived critical interest in numerous psychedelic compounds’ novel therapeutic applications. Of these, Psilocybe Cubensis (more commonly known as the “magic mushroom”) has gained much attention recently for it at psychoactive doses (sufficient to produce a “trip”) as well the purported benefits of regularly ingesting much lower doses or “microdoses.” Even more impressive is the potential synergistic nature recognized as a “mycological entourage effect” of sorts (like the “entourage effect” exhibited by cannabis) achievable by combining, i.e., “stacking” magic mushrooms with other medicinal types such as Lion’s Mane and other compounds to boost activity. World-renowned mycologist Paul Stamets touts this method of optimization. Dr. Stamets is preparing to launch a nootropic supplement for micro-dosers, which he hopes will catalyze our next “leap in human consciousness.”

As research into psychedelic substances such as psilocybin and their applications at low dosages continues, this guide will explore the potential impact of the novel Stamets micro-dosing “stack” from a historical context in conjunction with evidence based reviews.




Microdosing Psilocybe cubensis magic mushrooms shroom psychedelic

What is Microdosing?

Microdosing is the practice of ingesting a below-threshold, sub-perceptive dosage of a hallucinogenic substance, sometimes in tandem with other compounds to create a synergistic effect. At these levels, these compounds’ psychoactive effects are generally too subtle to be detected by the users’ conscious awareness, yet are still reported to enhance the senses and provide numerous other physical and mental benefits.[1] For psilocybin, a tenth of a gram still facilitates positive effects, and for LSD, around ten micrograms seem to be enough to exhibit positive physical and mental effects as well. Currently, clinical research into micro-dosing psychedelic compounds is limited and ongoing. Ultimately, the volume of anecdotal reporting continues to rise while supported by the established benefits studied at full doses or “trip doses” (a dose sufficiently high enough to produce the characteristic hallucinogenic/entheogenic effects of that particular psychedelic compound). Thus, the available evidence warrants large-scale, double-blind, placebo-controlled randomized trials to uncover and establish the physiological and psychological benefits microdosing has to offer.

Microdosing psilocybe cubensis shrooms mushrooms

The “Stoned Ape Theory” In Relation To Microdosing and Human Brain Development:

The recent spike in popularity in micro-dosing with psychedelic compounds can be linked to Silicon Valley techies who stand on the shoulders of giants. In fact, early humans may indeed have been consuming small quantities (or “microdoses”) of magic mushrooms to possibly improve their eyesight, gain extra energy, and increase their stamina when hunting. In reality, this type of optimization is the foundation for the “Stoned Ape Theory.” Posited by the renowned brothers, Dennis and Terrence McKenna, that argues early humans could make their dramatic “leap in consciousness” due to the positive effects on neurogenesis and neural plasticity exhibited by various mushrooms—especially psychedelic ones. The world-renowned mycologist Paul Stamets states that while the theory is more accurately a “hypothesis” since it lacks empirical evidence to back it up, there is significant merit to it even though we cannot test it. The current practice of Silicon Valley techs microdosing to reportedly ramp up their productivity, creativity, and overall enhance their cognition parallels the “Stoned Ape Theory,” whereby early humans used low doses of psilocybin to improve their hunting abilities. While the connection between mushrooms and their relationship to human evolution runs deep and spans millennia, their more recent emergence of psilocybin in the West can be partially attributed to prominent figures like Robert Wasson that sought out the magic mushroom and detailed their experiences for the Western audience.

microdosing psilocybin psilocybe cubensis psychedelic science

Robert Wasson: How Psilocybin Made Its Way to the West

In this discussion, it would be remiss not to briefly consider the first Westerner to share his experience of consuming Psilocybe Cubensis and the impact it had on raising awareness about the mushroom in the Western world. Thus, the prominent VP of Public Relations at J.P. Morgan & Co., Robert Wasson, and his role in sharing his firsthand experience in consuming psilocybin shrooms to the West through his encounters with Mazatec shamans cannot be understated.


Through his course of CIA funded research, the accomplished ethnomycologist and author made significant strides in raising awareness of the psilocybin mushroom’s powerful therapeutic potential. In his 1957 photo essay “Seeking the Magic Mushroom,” Wasson describes his fateful 1955 journey to Oaxaca, Mexico, where he partook in a sacred Mazatec ritual where he consumed the mushroom with indigenous tribesmen in the ceremony. Replete with photos from the famous Allan Richardson of several mushroom species identified by the then director of the French National Museum of History, Roger Heim, Wasson became one of the first Westerners to share a firsthand account of the profound experience using the sacred mushroom with the Western world. Wasson’s account was powerful and compelling, influencing iconic figures in the psychedelic movement to travel to Mexico searching for this mushroom, including the legendary and estranged Harvard Professor, Dr. Timothy Leary.[2]


These events would ultimately snowball into the beginnings of groundbreaking psychedelic research into these compounds, which, after a painful period of prohibition, are finally beginning to resurface in our world today.

microdosing psilocybin psilocybe cubensis psychedelic science

A Note About Current Psychedelic Research

 Indigenous cultures have aligned their traditions around the powerful potential of medicinal mushrooms for millennia, while scientific research is finally starting to catch up. Some of the most promising research revolves around the ability of psychedelics like psilocybin to promote functional and structural neuronal plasticity (e.g., the ability of the brain to form new neural connections is heightened).[3] Specifically, psilocybin alone (the active compound in magic mushrooms) has been shown to cause neurogenesis in the hippocampus, a part of the brain integral to learning and memory.[4]


Furthermore, psilocybin has been shown to modulate the brain’s parts that process fear, which is likely responsible for the remarkable effects seen in patients with life-threatening cancer who overcome their fear of death.[5] Such evidence regarding the neurological benefits of these mushrooms certainly offers support to the “Stoned Ape Theory” as it is possible the enhanced emotional intelligence and reduced fear response garnered from taking such mushrooms may have catalyzed the formation of leaders among groups of early humans, helping the species advance over other species in the animal kingdom.


Today, current research shows immense promise and clinical utility of psilocybin in the treatment of mental health.[6] A first of its kind, the John Hopkins University Center for Psychedelic Research, has been a pioneering force in psilocybin’s efficacy in treating various neurological disorders.[7] These studies show remarkable effects from large doses of psilocybin, but what about microdosing? Studies are limited, but anecdotal reports and self-reporting from the first clinical trial are indeed very promising.

microdosing psilocybin psilocybe cubensis psychedelic science

A Clinical Snapshot of Microdosing Today

 Today, everyone from the pioneering Silicon Valley techs to spiritualists and patients suffering from various mental health conditions is looking to psychedelics and microdosing in particular, to improve their lives. While clinical research is still in the early days for microdosing studies, trials are underway. Early results show positive reported results from participants; however, they were unrelated to the observed pattern of reported outcomes.[8]


Notably, another 2019 study stated that subjects who were microdosing reported “improved mood (26.6%) and focus (14.8%), and in terms of challenging outcomes, such as physiological discomfort (18.0%) and increased anxiety (6.7%).” [1] Combined with the available research regarding “trip” doses, this plethora of positive anecdotal and subject reported data about microdosing strongly calls for large scale, gold-standard clinical trials into the clinical utility of microdosing psychedelics.


Paul Stamets is undoubtedly one to get behind the massive potential microdosing psilocybin for humanity with his newly patented psilocybin microdosing “stack.”


Read: Can Microdosing Magic Mushrooms Help My Anxiety? An Evidence-Based Review 

microdosing psilocybin psilocybe cubensis psychedelic science

Paul Stamets on “Stacking” Supplements for an Optimal Microdosing Experience

Microdosing psilocybe cubensis shrooms mushrooms

While different protocols for “stacking”, or the use of different substances to synergize and promote the beneficial properties of complementary compounds in a formula, exist, a true focus should be placed on psilocybin. In particular, a recently patented formula by Paul Stamets calls for the addition of both Lion’s Mane mushroom and niacin, or vitamin B3 to create, in essence, a mycological entourage effect between the medicinal mushrooms and an overall synergy among the compounds in the formula.


Lion’s Mane is a medicinal (non-hallucinogenic) mushroom which has legitimate, neuroprotective qualities, promotes the production of nerve growth factor, and is considered a nootropic within brain-hacking communities, aiding in the relief of depression and anxiety.[9] Interestingly, the pharmacological action of Psilocybe cubensis (“magic mushrooms”) is such that it substitutes for serotonin (5HT) in the brain and acts as a more effective neurotransmitter thus activating neurogenesis, forming new neurons and subsequently, new pathways of knowledge.[4] Indeed, Stamets advocates for the benefits of this combination, claiming it optimal in achieving maximal benefits from the mushrooms.

The Stamets stack has been touted to be effective in improving cognition and performance by many, with many compelling accounts available on the internet. Nootropic experts from across the globe are touting the benefits of the Stamets stack, stating various remarkable benefits, including its ability to stimulate myelin production–a key factor in nerve cell growth. Indeed, the benefits of the Stamets stack go beyond performance enhancement and have serious implications on its ability to heal neurodegenerative disorders as well.

microdosing psilocybin psilocybe cubensis psychedelic science

The “Mycological Entourage Effect” Expanded & The Role of Niacin in Stamets’ “Stack”

In combination with the neurogenerative properties of Psilocybe cubensis, the Lion’s Mane (Hericium Erinaceus) contained in the Stamets stack helps to create a synergistic interaction that helps to boost the benefits of both substances (similar to the “entourage effect” of the Cannabis plant). The addition of niacin, as per Stamets’ suggestion, is two-pronged. The first benefit is associated with its propensity to create a “flush”—at around a 100mg dosage, the body will visibly become red and substantially itchy, as well.


This is borderline uncomfortable but tends to last only from ten to twenty minutes. The flush is a stimulation of the nervous system’s peripheral nerves and a critical component of the Stamets stack. Additionally, the stacked Lion’s Mane arguably boosts the overall neurogenerative effects. If this eventually becomes a mainstream, prescribed type of medication (Stamets is generally one to look ahead toward the realm of patentability while also considering safety and pragmatism), the niacin would discourage abuse. If someone were looking to get high, taking enough of these combined, encapsulated stacks would equate to a very high niacin dosage, causing much more significant overall discomfort. Clearly, Paul Stamets has thought ahead about the mainstream applications of his renowned Stamets stack. 

Microdosing psilocybe cubensis shrooms mushrooms

More Research Into Microdosing Is Currently Underway

The benefits of microdosing are still being studied. However, users often report an increased level of empathy, anxiolytic effects, and a disruption of regular thought loops, leading to increased creativity and the ability to tackle problems from a novel perspective. This element of novelty seems to be enticing Silicon Valley, with the industry of computer coding implementing microdosing to view coding difficulties from new perspectives. Additionally, artists of all mediums are at the forefront, using these substances to add new dimensions to their artistic endeavors.


Future Implications of Psilocybin Microdosing on the World at Large

 With the relaxing legality surrounding psychedelics, microdosing is becoming a legitimate means to personally address behavioral issues, boost creativity, add increased levels of compassion to human interaction, and challenge societal norms. The research is detailed that magic mushrooms have profound neurological effects that have been studied and found to be therapeutic (such as that of neurogenesis and rewiring the fear pathway). With the renowned mycologist Stamets aiming to launch his Microdosing psilocybin “stack” as a mainstream supplement to benefit the masses, could the world at large indeed be on the brink of making a “quantum leap in consciousness,” as described by Stamets himself? This clinical biologist and science writer certainly hopes so and is eager to see what the new research uncovers. Until then, citizens of the US and those of the world add to the anecdotal evidence that microdosing has real, legitimate benefits that should not be ignored but instead embraced and celebrated.

For those in the know about Psilocybin, we invite you to our upcoming Mushroom Conference (MushCon for short)! Tickets are available here: come join us and enjoy expert insight from those leading the psychedelic renaissance.

Works Cited


  1.     Anderson, T. et al. Psychedelic microdosing benefits and challenges: an empirical codebook. Harm. Reduct. J.16, (2019).
  2.     Hippies Flocking to. Mexico for Mushroom ‘Trips’ – The New York Times. https://www.nytimes.com/1970/07/23/archives/hippies-flocking-to-mexico-for-mushroom-trips.html.
  3.     Ly, C. et al. Psychedelics Promote Structural and Functional Neural Plasticity. Cell Rep. 23, 3170–3182 (2018).
  4.     Catlow, B. J., Song, S., Paredes, D. A., Kirstein, C. L. & Sanchez-Ramos, J. Effects of psilocybin on hippocampal neurogenesis and extinction of trace fear conditioning. Exp. Brain Res. 228, 481–491 (2013).
  5.     Griffiths, R. R. et al. Psilocybin produces substantial and sustained decreases in depression and anxiety in patients with life-threatening cancer: A randomized double-blind trial. J. Psychopharmacol. Oxf. Engl. 30, 1181–1197 (2016).
  6.     Daniel, J. & Haberman, M. Clinical potential of psilocybin as a treatment for mental health conditions. Ment. Health Clin. 7, 24–28 (2018).
  7.     Johns Hopkins launches center for psychedelic research | Hub. https://hub.jhu.edu/2019/09/04/hopkins-launches-psychedelic-center/.
  8.     Polito, V. & Stevenson, R. J. A systematic study of microdosing psychedelics. PLoS ONE 14, (2019).
  9. Li, I.-C. et al. Neurohealth Properties of Hericium erinaceus Mycelia Enriched with Erinacines. Behav. Neurol.2018, (2018).

Gaurav Dubey

Gaurav Dubey

Gaurav Dubey is a dynamic clinical biologist, evidence-based branding professional and entrepreneur with a passion for scientific writing and traveling the world. He earned a dual bachelor’s degree in biology and philosophy from the University of Miami in 2011 and his Master’s in Biotechnology from Rush University Medical Center in 2015. Gaurav was recently published as lead author in the world's leading transplant journal, The Journal of Heart and Lung Transplantation, for a study investigating second-time lung re-transplantation. He is passionate about psychedelic medicine and recently accepted a position as content editor at Microdose, an opportunity he is very excited about. He is also the Founder & President of Karmik, LLC, an evidence-based branding firm for extraordinary life science brands.